Integrated Plant Genetics | Citrus Canker In-Depth

Citrus Canker In-Depth

I. Economic Hosts

II. Pathogens

III. Disease

IV. Symptoms and Signs

V. Isolation:

X. citri strains may be isolated and cultured from all affected plant tissues by commonly used methods. Lesions are excised with a scalpel or razor, washed with tap water, surface sterilized for 3 minutes in a 10% dilution of commercial hypochlorite bleach, rinsed and sectioned. The water-soaked tissue at the lesion margin is dragged across a sterile agar medium containing 50 µg/ml kasugamycin. X. citri strains grow well on various nutrient agar media, and the following works well: 0.5% tryptone, 0.3% yeast extract, 0.09% CaCl2, 0.05% K2HPO4 and 1.5% agar in tap water, pH 7.2. We typically buffer this medium with 100mM morpholinopropane sulfate (MOPS) plus micronutrients (TY-MOPS) [7]. Yellow mucoid colonies should appear within 48 hours.

X. campestris pv. aurantifolii strains are reportedly difficult to isolate and culture directly from citrus tissue; these strains may be cultured initially on 1% sucrose, 0.5% peptone, 0.05% K2HPO4, 0.03% MgSO4 and Difco purified agar [2]. After initial culturing, however, these strains appear to adapt to other media and may be routinely cultured on TY-MOPS or other nutrient media.

VI. Identification

VII. Pathogenicity

VIII. Storage of Organism

IX. Reported Host Range

X. Geographical Range and Spread

XI. Suggested Taxonomic Keys

XII. References

For general inquiries, please send e-mail to info@ipgenetics.com. For web site errors or content issues, please e-mail webmaster@ipgenetics.com.